Electrochemiluminescence nanoimmunosensor for CD63 protein using a carbon nanochips/iron oxide/nafion-nanocomposite modified mesoporous carbon interface

Adhikari, Juthi and Rizwan, Mohammad and Dennany, Lynn and Ahmed, Minhaz Uddin (2021) Electrochemiluminescence nanoimmunosensor for CD63 protein using a carbon nanochips/iron oxide/nafion-nanocomposite modified mesoporous carbon interface. Measurement, 170. 108755. ISSN 1873-412X (https://doi.org/10.1016/j.measurement.2020.108755)

[thumbnail of Adhikari-etal-Measurement-2020-Electrochemiluminescence-nanoimmunosensor-for-CD63-protein-using-a-carbon]
Preview
 Text. Filename: Adhikari_etal_Measurement_2020_Electrochemiluminescence_nanoimmunosensor_for_CD63_protein_using_a_carbon.pdf
Accepted Author Manuscript
License: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 logo
Download (1MB)| Preview

Abstract

The detection of extracellular vesicles, or exosomes are important mediators in intercellular communication and often play a role in cancer progression. CD63 is a key exosomal protein due to its distinctive cellular functions and association with many cancers. This describes a label-free electrochemiluminescence (ECL) nanoimmunosensor for the detection of CD63 protein over mesoporous carbon screen-printed electrode (MC-SPE) modified with novel nanocomposite of carbon nanochips (CNCs), iron oxide (Fe3O4) and nafion (NAF) . Fourier-transform infrared spectroscopy and field emission scanning electron microscopy were used to analyse nanocomposite. All the analytical performance of fabricated CD63 immunosensor were conducted applying ECL. In spite of the simple fabrication strategies utilized, the fabricated immunosensor showcased a broad linear range to detect CD63 from 100 fg mL-1 to 10 ng mL-1, with a limit of detection of 100 fg mL-1, excellent selectivity, interference-resistance capability and potential to detect CD63 in real clinical samples.

ORCID iDs

Adhikari, Juthi, Rizwan, Mohammad, Dennany, Lynn ORCID logoORCID: https://orcid.org/0000-0002-5481-1066 and Ahmed, Minhaz Uddin;
  • Item type:Article
    ID code:74740
    Dates:
    Date
    Event
    31 January 2021
    Published
    23 November 2020
    Published Online
    16 November 2020
    Accepted
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:30 Nov 2020 16:25
    Last modified:21 Nov 2024 01:19
    URI:https://strathprints.strath.ac.uk/id/eprint/74740
CORE (COnnecting REpositories)