Investigation of the effect of channel structure and flow rate on on-chip bacterial lysis

Norouz Dizaji, Araz and Ozturk, Yasin and Ghorbanpoor, Hamed and Cetak, Ahmet and Akcakoca, Iremnur and Kocagoz, Tanil and Avci, Huseyin and Corrigan, Damion and Dogan Guzel, Fatma (2020) Investigation of the effect of channel structure and flow rate on on-chip bacterial lysis. IEEE Transactions on NanoBioscience. ISSN 1536-1241 (https://doi.org/10.1109/TNB.2020.3031346)

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Abstract

Successful lysis of cells/microorganisms is a key step in the sample preparation in fields like molecular biology, bioengineering, and biomedical engineering. This study therefore aims to investigate the lysis of bacteria on-chip and its dependence on both microfluidic channel structure and flow rate. Effects of temperature on lysis on-chip were also investigated. To perform these investigations, three different microfluidic chips were designed and produced (straight, zigzag and circular configurations), while the length of the channels were kept constant. As an exemplary case, Mycobacterium smegmatis was chosen to represent the acid-fast bacteria. Bacterial suspensions of 1.5 McFarland were injected into the chips at various flow rates (0.6-8 ll/min) either at room temperature or 500 C. In order to understand the on-chip lysis performance fully, off-chip experiments were carried out at durations which are equal to those bacteria spent in the channel from inlet to the outlet at different flow rates. We also performed COMSOL multiphysics program simulations to evaluate further the effect of the applied parameters. As a result, we found that the structure and the flow rate do not affect lysis over all in all investigated channel types, however on-chip experiments at room temperature produced more effective lysis compared to the on-chip and the off-chip samples performed at higher temperatures. Interestingly on-chip experiments at higher tempratures do not result in effective lysis.

ORCID iDs

Norouz Dizaji, Araz, Ozturk, Yasin, Ghorbanpoor, Hamed, Cetak, Ahmet, Akcakoca, Iremnur, Kocagoz, Tanil, Avci, Huseyin, Corrigan, Damion ORCID logoORCID: https://orcid.org/0000-0002-4647-7483 and Dogan Guzel, Fatma;
  • Item type:Article
    ID code:74345
    Dates:
    Date
    Event
    15 October 2020
    Published
    15 October 2020
    Published Online
    13 October 2020
    Accepted
    Notes:© 2020 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting /republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
    Subjects:Technology > Engineering (General). Civil engineering (General) > Bioengineering
    Department:Faculty of Engineering > Biomedical Engineering
    Depositing user: Pure Administrator
    Date deposited:22 Oct 2020 16:11
    Last modified:11 Nov 2024 12:52
    URI:https://strathprints.strath.ac.uk/id/eprint/74345
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