Tracking insulin glycation in real time by time-resolved emission spectroscopy

Chung, Li Hung C. and Birch, David J. S. and Vyshemirsky, Vladislav and Ryadnov, Maxim G. and Rolinski, Olaf J. (2019) Tracking insulin glycation in real time by time-resolved emission spectroscopy. Journal of Physical Chemistry B, 123 (37). pp. 7812-7817. ISSN 1520-6106 (https://doi.org/10.1021/acs.jpcb.9b06363)

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Abstract

The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.

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