Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra

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Chung, Li Hung C and Birch, David J S and Vyshemirsky, Vladislav and Bella, Angelo and Ryadnov, Maxim G and Rolinski, Olaf J (2019) Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra. Methods and Applications in Fluorescence, 7 (3). 035003. ISSN 2050-6120 (https://doi.org/10.1088/2050-6120/ab1985)

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Abstract

The excited-state kinetics of the fluorescence of tyrosine in a de novo protein fibrillogenesis model was investigated as a potential tool for monitoring protein fibre formation and complexation with glucose (glycation). In stark contrast to insulin the time-resolved emission spectra (TRES) recorded over the period of 700 hours in buffered solutions of the model with and without glucose revealed no apparent changes in Tyr fluorescence responses. This indicates the stability of the model and provides a measurement-supported basis for its use as a reference material in fluorescence studies of protein aggregation.

ORCID iDs

Chung, Li Hung C ORCID logoORCID: https://orcid.org/0000-0002-1043-3227, Birch, David J S ORCID logoORCID: https://orcid.org/0000-0001-6400-1270, Vyshemirsky, Vladislav, Bella, Angelo, Ryadnov, Maxim G and Rolinski, Olaf J ORCID logoORCID: https://orcid.org/0000-0002-7838-779X;
  • Item type:Article
    ID code:68773
    Dates:
    Date
    Event
    16 May 2019
    Published
    15 April 2019
    Accepted
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:09 Jul 2019 08:11
    Last modified:11 Nov 2024 12:21
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/68773
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