Single-molecule localization microscopy analysis with ImageJ

van de Linde, Sebastian (2019) Single-molecule localization microscopy analysis with ImageJ. Journal of Physics D: Applied Physics, 52 (20). 203002. ISSN 0022-3727 (https://doi.org/10.1088/1361-6463/ab092f)

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Abstract

ImageJ is a versatile and powerful tool for quantitative image analysis in microscopy. It is open-source software, platform-independent and enables students and researchers to obtain an easy but thorough introduction into image analysis. Especially the image processing package Fiji is a valuable and powerful extension of ImageJ. Several plugins and macros for single-molecule localization microscopy (SMLM) have been developed during the last decade. These novel tools cover the steps from single-molecule localization and image reconstruction to SMLM data postprocessing such as density analysis, image registration or resolution estimation. This article describes how ImageJ/Fiji can be used for image analysis, reviews existing extensions for SMLM, and aims at introducing and motivating novices and advanced SMLM users alike to explore the possibilities of ImageJ/Fiji for automated and quantitative data analysis.

  • Item type:Article
    ID code:67360
    Dates:
    Date
    Event
    13 March 2019
    Published
    21 February 2019
    Published Online
    21 February 2019
    Accepted
    1 October 2018
    Submitted
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:19 Mar 2019 12:40
    Last modified:09 Apr 2024 00:35
    URI:https://strathprints.strath.ac.uk/id/eprint/67360
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