Wide-field multiphoton imaging through scattering media without correction

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Escobet-Montalbán, Adrià and Spesyvtsev, Roman and Chen, Mingzhou and Saber, Wardiya Afshar and Andrews, Melissa and Herrington, C. Simon and Mazilu, Michael and Dholakia, Kishan (2018) Wide-field multiphoton imaging through scattering media without correction. Science Advances, 4 (10). ISSN 2375-2548 (https://doi.org/10.1126/sciadv.aau1338)

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Abstract

Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.

  • Item type:Article
    ID code:65832
    Dates:
    Date
    Event
    2 October 2018
    Published
    7 September 2018
    Accepted
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:18 Oct 2018 11:06
    Last modified:26 Nov 2024 12:38
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/65832
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