The therapeutic effect of anti-CD52 treatment in murine experimental autoimmune encephalomyelitis is associated with altered IL-33 and ST2 expression levels

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Barbour, Mark and Wood, Rachel and Hridi, Shehla U and Wilson, Chelsey and McKay, Grant and Bushell, Trevor J and Jiang, Hui-Rong (2018) The therapeutic effect of anti-CD52 treatment in murine experimental autoimmune encephalomyelitis is associated with altered IL-33 and ST2 expression levels. Journal of Neuroimmunology. pp. 1-33. ISSN 0165-5728 (https://doi.org/10.1016/j.jneuroim.2018.02.012)

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Abstract

Experimental autoimmune encephalomyelitis (EAE) mice were administered with murine anti-CD52 antibody to investigate its therapeutic effect and whether the treatment modulates IL-33 and ST2 expression. EAE severity and central nervous system (CNS) inflammation were reduced following the treatment, which was accompanied by peripheral T and B lymphocyte depletion and reduced production of various cytokines including IL-33, while sST2 was increased. In spinal cords of EAE mice, while the number of IL-33+ cells remained unchanged, the extracellular level of IL-33 protein was significantly reduced in anti-CD52 antibody treated mice compared with controls. Furthermore the number of ST2+ cells in the spinal cord of treated EAE mice was downregulated due to decreased inflammation and immune cell infiltration in the CNS. These results suggest that treatment with anti-CD52 antibody differentially alters expression of IL-33 and ST2, both systemically and within the CNS, which may indicateIL-33/ST2 axis is involved in the action of the antibody in inhibiting EAE.

ORCID iDs

Barbour, Mark ORCID logoORCID: https://orcid.org/0000-0003-1547-1289, Wood, Rachel ORCID logoORCID: https://orcid.org/0000-0001-6366-8508, Hridi, Shehla U ORCID logoORCID: https://orcid.org/0000-0001-7906-6763, Wilson, Chelsey ORCID logoORCID: https://orcid.org/0000-0002-1235-1110, McKay, Grant, Bushell, Trevor J ORCID logoORCID: https://orcid.org/0000-0003-4145-9670 and Jiang, Hui-Rong;
CORE (COnnecting REpositories)