Biocatalytic self-assembly using reversible and irreversible enzyme immobilization

Conte, M. P. and Lau, K. H. A. and Ulijn, R. V. (2017) Biocatalytic self-assembly using reversible and irreversible enzyme immobilization. ACS Applied Materials and Interfaces, 9 (4). pp. 3266-3271. ISSN 1944-8252 (https://doi.org/10.1021/acsami.6b13162)

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Abstract

Biocatalytic control of molecular self-assembly provides an effective approach for developing smart biomaterials, allowing versatile enzyme-mediated tuning of material structure and properties as well as biomedical applications. We functionalized surfaces with bioinspired polydopamine and polyphenol coatings to study the effects of enzyme surface localization and surface release on the self-assembly process. We show how these coatings could be conveniently used to release enzymes for bulk gelation as well as to irreversibly immobilize enzymes for localizing the self-assembly to the surface. The results provide insights to the mode of action of biocatalytic self-assembly relevant to nanofabrication and enzyme-responsive materials.

ORCID iDs

Conte, M. P. ORCID logoORCID: https://orcid.org/0000-0001-8706-4901, Lau, K. H. A. ORCID logoORCID: https://orcid.org/0000-0003-3676-9228 and Ulijn, R. V. ORCID logoORCID: https://orcid.org/0000-0001-7974-3779;
  • Item type:Article
    ID code:59560
    Dates:
    Date
    Event
    1 February 2017
    Published
    12 January 2017
    Published Online
    12 January 2017
    Accepted
    16 October 2016
    Submitted
    Notes:This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials and Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acsami.6b13162
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:24 Jan 2017 10:14
    Last modified:20 Nov 2024 01:13
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/59560
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