Bioprospecting from marine sediments of New Brunswick, Canada : exploring the relationship between total bacterial diversity and actinobacteria diversity

Duncan, Katherine and Haltli, Bradley and Gill, Krista A and Kerr, Russell G (2014) Bioprospecting from marine sediments of New Brunswick, Canada : exploring the relationship between total bacterial diversity and actinobacteria diversity. Marine Drugs, 12 (2). pp. 899-925. ISSN 1660-3397 (https://doi.org/10.3390/md12020899)

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Abstract

Actinomycetes are an important resource for the discovery of natural products with therapeutic properties. Bioprospecting for actinomycetes typically proceeds without a priori knowledge of the bacterial diversity present in sampled habitats. In this study, we endeavored to determine if overall bacterial diversity in marine sediments, as determined by 16S rDNA amplicon pyrosequencing, could be correlated with culturable actinomycete diversity, and thus serve as a powerful tool in guiding future bioprospecting efforts. Overall bacterial diversity was investigated in eight marine sediments from four sites in New Brunswick, Canada, resulting in over 44,000 high quality sequences (x = 5610 per sample). Analysis revealed all sites exhibited significant diversity (H' = 5.4 to 6.7). Furthermore, statistical analysis of species level bacterial communities (D = 0.03) indicated community composition varied according to site and was strongly influenced by sediment physiochemical composition. In contrast, cultured actinomycetes (n = 466, 98.3% Streptomyces) were ubiquitously distributed among all sites and distribution was not influenced by sediment composition, suggesting that the biogeography of culturable actinomycetes does not correlate with overall bacterial diversity in the samples examined. These actinomycetes provide a resource for future secondary metabolite discovery, as exemplified by the antimicrobial activity observed from preliminary investigation.

ORCID iDs

Duncan, Katherine ORCID logoORCID: https://orcid.org/0000-0002-3670-4849, Haltli, Bradley, Gill, Krista A and Kerr, Russell G;
  • Item type:Article
    ID code:57824
    Dates:
    Date
    Event
    13 February 2014
    Published
    21 January 2014
    Accepted
    Subjects:Medicine > Pharmacy and materia medica
    Department:UNSPECIFIED
    Depositing user: Pure Administrator
    Date deposited:16 Sep 2016 10:59
    Last modified:11 Nov 2024 11:29
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/57824
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