Electrochemiluminescence platform for the detection of C-reactive proteins : application of recombinant antibody technology to cardiac biomarker detection

O'Reilly, Emmet J. and Conroy, Paul J. and Hearty, Stephen and Keyes, Tia E. and O'Kennedy, Richard and Forster, Robert J. and Dennany, Lynn (2015) Electrochemiluminescence platform for the detection of C-reactive proteins : application of recombinant antibody technology to cardiac biomarker detection. RSC Advances, 5 (83). pp. 67874-67877. ISSN 2046-2069 (https://doi.org/10.1039/C5RA08450D)

[thumbnail of O'Reilly-etal-RSCA2015-Electrochemiluminescence-platform-for-the-detection-of-C-reactive-proteins]
Preview
 Text. Filename: O_Reilly_etal_RSCA2015_Electrochemiluminescence_platform_for_the_detection_of_C_reactive_proteins.pdf
Accepted Author Manuscript
Download (661kB)| Preview

Abstract

This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

ORCID iDs

O'Reilly, Emmet J., Conroy, Paul J., Hearty, Stephen, Keyes, Tia E., O'Kennedy, Richard, Forster, Robert J. and Dennany, Lynn ORCID logoORCID: https://orcid.org/0000-0002-5481-1066;
CORE (COnnecting REpositories)