Pulsed electric field treatment on Saccharomyces cerevisiae using different waveforms

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Qin, S. and Timoshkin, I. V. and MacLean, M. and Wilson, M. P. and Given, M. J. and Wang, T. and Anderson, J. G. and MacGregor, S. J. (2015) Pulsed electric field treatment on Saccharomyces cerevisiae using different waveforms. IEEE Transactions on Dielectrics and Electrical Insulation, 22 (4). pp. 1841-1848. ISSN 1070-9878 (https://doi.org/10.1109/TDEI.2015.005152)

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Abstract

Pulsed electric field (PEF) treatment can be used for non-thermal inactivation of microorganisms. The aim of this paper is to investigate PEF treatment of yeast, Saccharomyces cerevisiae, using three different field waveforms: square; non-oscillating exponential and oscillating exponential. The PEF system used in this paper consists of a pulsed power supply and a parallel-plane metallic electrodes treatment cell located in an air-pressurised chamber. PEF treatment of the yeast was conducted using electric field impulses with magnitudes of 67 kV/cm and 80 kV/cm. The efficacy of the PEF treatment for inactivation of the yeast cells was assessed by comparison of the PEF-treated and untreated yeast populations. Results showed that 3-log10 reduction in the yeast population can be achieved with 100 impulses using all tested waveforms. Amongst all three tested waveforms non-oscillating exponential impulses demonstrated improved PEF performance. The effect of duration of treatment and peak magnitude of the field on the PEF process is discussed.

ORCID iDs

Qin, S. ORCID logoORCID: https://orcid.org/0000-0001-8616-6121, Timoshkin, I. V. ORCID logoORCID: https://orcid.org/0000-0002-0380-9003, MacLean, M. ORCID logoORCID: https://orcid.org/0000-0001-5750-0397, Wilson, M. P. ORCID logoORCID: https://orcid.org/0000-0003-3088-8541, Given, M. J. ORCID logoORCID: https://orcid.org/0000-0002-6354-2486, Wang, T. ORCID logoORCID: https://orcid.org/0000-0003-3054-0772, Anderson, J. G. ORCID logoORCID: https://orcid.org/0000-0003-4151-1619 and MacGregor, S. J. ORCID logoORCID: https://orcid.org/0000-0002-0808-585X;
  • Item type:Article
    ID code:53888
    Dates:
    Date
    Event
    11 August 2015
    Published
    9 April 2015
    Accepted
    Notes:© 2015 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
    Subjects:Technology > Electrical engineering. Electronics Nuclear engineering
    Department:Faculty of Engineering > Electronic and Electrical Engineering
    Depositing user: Pure Administrator
    Date deposited:28 Jul 2015 08:26
    Last modified:12 Dec 2024 03:10
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/53888
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