New antifouling platform characterized by single-molecule imaging

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Ryu, Ji Young and Song, In Taek and Lau, K. H. Aaron and Messersmith, Phillip B. and Yoon, Tae-Young and Lee, Haeshin (2014) New antifouling platform characterized by single-molecule imaging. ACS Applied Materials and Interfaces, 6 (5). pp. 3553-3558. ISSN 1944-8252 (https://doi.org/10.1021/am4057387)

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Abstract

Antifouling surfaces have been widely studied for their importance in medical devices and industry. Antifouling surfaces mostly achieved by methoxy-poly(ethylene glycol) (mPEG) have shown biomolecular adsorption less than 1 ng/cm2 which was measured by surface analytical tools such as surface plasmon resonance (SPR) spectroscopy, quartz crystal microbalance (QCM), or optical waveguide lightmode (OWL) spectroscopy. Herein, we utilize a single-molecule imaging technique (i.e., an ultimate resolution) to study antifouling properties of functionalized surfaces. We found that about 600 immunoglobulin G (IgG) molecules are adsorbed. This result corresponds to ∼5 pg/cm2 adsorption, which is far below amount for the detection limit of the conventional tools. Furthermore, we developed a new antifouling platform that exhibits improved antifouling performance that shows only 78 IgG molecules adsorbed (∼0.5 pg/cm2). The antifouling platform consists of forming 1 nm TiO2 thin layer, on which peptidomimetic antifouling polymer (PMAP) is robustly anchored. The unprecedented antifouling performance can potentially revolutionize a variety of research fields such as single-molecule imaging, medical devices, biosensors, and others.

ORCID iDs

Ryu, Ji Young, Song, In Taek, Lau, K. H. Aaron ORCID logoORCID: https://orcid.org/0000-0003-3676-9228, Messersmith, Phillip B., Yoon, Tae-Young and Lee, Haeshin;
CORE (COnnecting REpositories)