Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2

McConnell, G. and Riis, E. (2004) Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2. Physics in Medicine and Biology, 49 (20). pp. 4757-4763. ISSN 0031-9155 (http://dx.doi.org/10.1088/0031-9155/49/20/007)

Abstract

We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.

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• Item metadata

  Item type:	Article
  ID code:	50
  Dates:	Date Event 2004 Published
  Subjects:	Science > Physics > Optics. Light
  Department:	Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Faculty of Science > Physics
  Depositing user:	Ms FM Breslin
  Date deposited:	12 Dec 2005
  Last modified:	08 Apr 2024 15:30
  URI:	https://strathprints.strath.ac.uk/id/eprint/50