Spermine-induced hybridization and charge inversion at the diffuse layer of a DNA-FET

De La Rica, Roberto and Fernandez-Sanchez, Cesar and Cecilia, Jimenez-Jorquera and Baldi, Antonio (2008) Spermine-induced hybridization and charge inversion at the diffuse layer of a DNA-FET. Journal of Physical Chemistry B, 112 (25). pp. 7614-7617. ISSN 1520-6106 (https://doi.org/10.1021/jp711348m)

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Abstract

Detection of DNA hybridization events by using field-effect transducers is limited by the electrolyte content of the medium. So far, DNA was thought to hybridize only in solutions with concentrated electrolytes. In these media, the interface between the transducer gate and the solution is reduced to a thin layer in close contact with the surface, and DNA is poorly detected. In the present work, this limitation is overcome by using spermine as screening polycation. Hybridization assays with polycation concentrations as low as 10 mu M are reported. This ensures that hybridization takes place at the diffuse layer of the interface. The reported results suggest a charge-inversion mechanism induced by spermine. A target sequence is real-time label-free detected in the range from 10 to 500 nM.

  • Item type:Article
    ID code:45762
    Dates:
    Date
    Event
    26 June 2008
    Published
    31 May 2008
    Published Online
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:08 Nov 2013 10:07
    Last modified:08 Apr 2024 20:55
    URI:https://strathprints.strath.ac.uk/id/eprint/45762
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