Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays

Ito, M. and Nakamura, F. and Baba, A. and Tamada, K. and Ushijima, H. and Lau, K. H. A. and Manna, A. and Knoll, W. (2007) Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays. Journal of Physical Chemistry C, 111 (31). pp. 11653-11662. ISSN 1932-7447 (https://doi.org/10.1021/jp070524m)

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Abstract

We have developed a new methodology to fabricate high-resolution DNA microarrays (2500 dots/cm(2)) in combination with microcontact printing (mu CP) and surface plasmon resonance (SPR) imaging. A novel COOH-terminated PEG-disulfide with para-carborane (p-carborane) was synthesized as an initiator chemically bound to a gold substrate for surface coupling reactions with NH2-terminated DNA. The hybridization between target DNA immobilized on gold nanoparticles and probe DNA arrayed on flat gold substrates was successfully demonstrated by SPR imaging, where nonspecific adsorption was not observed at the array background. The gold nanoparticles on the array give quite high contrast even at low surface coverage with gold nanoparticles (similar to 10%) by the enhancement effect of optical signals based on nanoscale phenomena. This enhancement effect can be well described by the simulation based on the Maxwell-Garnett theory for the effective dielectric constants and Fresnel's equation.

ORCID iDs

Ito, M., Nakamura, F., Baba, A., Tamada, K., Ushijima, H., Lau, K. H. A. ORCID logoORCID: https://orcid.org/0000-0003-3676-9228, Manna, A. and Knoll, W.;
  • Item type:Article
    ID code:45543
    Dates:
    Date
    Event
    2007
    Published
    19 July 2007
    Published Online
    Notes:M1 - 31 Times Cited: 35
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:01 Nov 2013 16:06
    Last modified:11 Nov 2024 10:32
    URI:https://strathprints.strath.ac.uk/id/eprint/45543
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