Enzyme-responsive hydrogel particles for the controlled release of proteins : designing peptide actuators to match payload
Thornton, Paul D. and Mart, Robert J. and Webb, Simon J. and Ulijn, Rein V. (2008) Enzyme-responsive hydrogel particles for the controlled release of proteins : designing peptide actuators to match payload. Soft Matter, 4 (4). pp. 821-827. ISSN 1744-6848 (https://doi.org/10.1039/b714750c)
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We report on enzyme-responsive hydrogel particles for the controlled release of proteins. Amino-functionalised poly(ethylene glycol acrylamide) (PEGA) hydrogel particles were functionalised with peptide actuators that cause charge-induced swelling and payload release when triggered enzymatically. Peptide-based actuators were designed to match the specificity of the target enzyme, while also matching the charge properties of the to-be released protein payload, thereby uniquely allowing for tuneable release profiles. Fluorescently labelled albumin and avidin, proteins of similar size but opposite charge, were released at a rate that was governed by the peptide actuator linked to the polymer carrier, offering a highly controlled release mechanism. Release profiles were analysed using a combination of fluorescence spectroscopy of the solution and two-photon fluorescence microscopy to analyse enzymatically triggered molecular events within hydrogel particles during the initial stages of release.
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Item type: Article ID code: 43956 Dates: DateEvent2008Published8 February 2008Published OnlineSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 30 May 2013 12:11 Last modified: 08 Apr 2024 20:31 URI: https://strathprints.strath.ac.uk/id/eprint/43956