Interfacing biodegradable molecular hydrogels with liquid crystals

Tools

Lin, I-Hsin and Birchall, Louise S. and Hodson, Nigel and Ulijn, Rein V. and Webb, Simon J. (2013) Interfacing biodegradable molecular hydrogels with liquid crystals. Soft Matter, 9 (4). pp. 1188-1193. ISSN 1744-6848 (https://doi.org/10.1039/c2sm27160e)

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Abstract

A self-assembled Fmoc-peptide hydrogel has been interfaced with a liquid crystal (LC) display to give an optical sensor for enzyme activity. An Fmoc-TL-OMe hydrogel was selected as it can be formed in situ by enzyme-mediated assembly with thermolysin, and undergoes enzyme-mediated diassembly upon subtilisin addition. This enzyme-responsive hydrogel provides a semi-rigid, highly hydrated and biocompatible environment that also holds the LC display in place. A dual layer design was developed, where a phospholipid-loaded upper gel layer was separated from the LC display by a phospholipid free lower layer. Subtilisin (0.15 mM) digested both layers to give a gel-to-sol transition after several hours that liberated the phospholipid and produced a light-to-dark optical change in the LC display. The optical response was dependent upon the gel-to-sol transition; elastase or common components of serum did not disassemble the Fmoc-TL-OMe hydrogel and did not give an optical response.

ORCID iDs

Lin, I-Hsin, Birchall, Louise S., Hodson, Nigel, Ulijn, Rein V. ORCID logoORCID: https://orcid.org/0000-0001-7974-3779 and Webb, Simon J.;
CORE (COnnecting REpositories)