Mammalian cell entry genes in Streptomyces may provide clues to the evolution of bacterial virulence
Clark, Laura and Seipke, Ryan and Prieto, Pilar and Willemse, Joost and van Wezel, G. and Hutchings, M.I. and Hoskisson, Paul (2013) Mammalian cell entry genes in Streptomyces may provide clues to the evolution of bacterial virulence. Scientific Reports, 3. 1109. ISSN 2045-2322 (https://doi.org/10.1038/srep01109)
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Abstract
Understanding the evolution of virulence is key to appreciating the role specific loci play in pathogenicity. Streptomyces species are generally non-pathogenic soil saprophytes, yet within their genome we can find homologues of virulence loci. One example of this is the mammalian cell entry (mce) locus, which has been characterised in Mycobacterium tuberculosis. To investigate the role in Streptomyces we deleted the mce locus and studied its impact on cell survival, morphology and interaction with other soil organisms. Disruption of the mce cluster resulted in virulence towards amoebae (Acanthamoeba polyphaga) and reduced colonization of plant (Arabidopsis) models, indicating these genes may play an important role in Streptomyces survival in the environment. Our data suggest that loss of mce in Streptomyces spp. may have profound effects on survival in a competitive soil environment, and provides insight in to the evolution and selection of these genes as virulence factors in related pathogenic organisms.
ORCID iDs
Clark, Laura, Seipke, Ryan, Prieto, Pilar, Willemse, Joost, van Wezel, G., Hutchings, M.I. and Hoskisson, Paul ORCID: https://orcid.org/0000-0003-4332-1640;-
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Item type: Article ID code: 42420 Dates: DateEvent23 January 2013PublishedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 19 Dec 2012 12:19 Last modified: 11 Nov 2024 10:18 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/42420