Fluorescence anisotropy of protein - gold nanoclusters

Tools

Li, Chenhao and Sutter, Jens and Birch, David and Chen, Yu; (2012) Fluorescence anisotropy of protein - gold nanoclusters. In: 2012 12th IEEE Conference on Nanotechnology. IEEE, New York. ISBN 9781467322003 (https://doi.org/10.1109/NANO.2012.6322067)

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Abstract

Red-emitting gold nanoclusters (BSA-Au-25) have been synthesized in Bovine Serum albumin and found to have a characteristic fluorescence lifetime of 1.25 mu s. Fluorescence anisotropy measurements of BSA-Au-25 reveal a dramatic increase in protein size as the pH is reduced from 7 to 3.1, consistent with the change of BSA from N form to E form. This study suggests the possibility of exploiting fluorescent protein-gold nanoclusters to probe protein conformational changes in response to the local environment.

ORCID iDs

Li, Chenhao, Sutter, Jens ORCID logoORCID: https://orcid.org/0000-0002-3782-6338, Birch, David ORCID logoORCID: https://orcid.org/0000-0001-6400-1270 and Chen, Yu;
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