Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

McConnell, G. and Smith, G.L. and Girkin, J.M. and Gurney, A.M. and Ferguson, A.I. (2003) Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source. Optics Letters, 28 (19). pp. 1742-1744. ISSN 0146-9592 (http://dx.doi.org/doi:10.1364/OL.28.001742)

Abstract

We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca 2+ indicator fura-2.

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• Item metadata

  Item type:	Article
  ID code:	38
  Dates:	Date Event 2003 Published
  Subjects:	Science > Physics > Optics. Light
  Department:	Faculty of Science > Physics > Institute of Photonics Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Physiology and Pharmacology Faculty of Science > Physics
  Depositing user:	Miss Jacqueline Miller
  Date deposited:	12 Dec 2005
  Last modified:	08 Apr 2024 15:26
  URI:	https://strathprints.strath.ac.uk/id/eprint/38