Dual triggering of DNA binding and fluorescence via photoactivation of a dinuclear ruthenium(II) arene complex

Magennis, Steven W. and Habtemariam, Abraha and Novakova, Olga and Henry, John B. and Meier, Samuel and Parsons, Simon and Oswald, Iain D. H. and Brabec, Viktor and Sadler, Peter J. (2007) Dual triggering of DNA binding and fluorescence via photoactivation of a dinuclear ruthenium(II) arene complex. Inorganic Chemistry, 46 (12). pp. 5059-5068. ISSN 1520-510X (https://doi.org/10.1021/ic062111q)

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Abstract

The dinuclear Ru-II arene complexes [{(eta(6)-arene)RuCl}(2)(mu-2,3-dpp)](PF6)(2), arene = indan (1), benzene (2), p-cymene (3), or hexamethylbenzene (4) and 2,3-dpp = 2,3-bis(2-pyridyl)pyrazine, have been synthesized and characterized. Upon irradiation with UVA light, complexes 1 and 2 readily underwent arene loss, while complexes 3 and 4 did not. The photochemistry of 1 was studied in detail. In the X-ray structure of [{(eta(6)-indan)RuCl}(2)(mu-2,3-dpp)](PF6)(2) (1), 2,3-dpp bridges two Ru-II centers 6.8529(6) A apart. In water, aquation of 1 in the dark occurs with replacement of chloride with biexponential kinetics and decay constants of 100 +/- 1 min(-1) and 580 +/- 11 min(-1). This aquation was suppressed by 0.1 M NaCl. UV or visible irradiation of 1 in aqueous or methanolic solution led to arene loss. The fluorescence of the unbound arene is similar to 40 times greater than when it is complexed. Irradiation of 1 also had a significant effect on its interactions with DNA. The DNA binding of 1 is increased after irradiation. The non-irradiated form of 1 preferentially formed DNA adducts that only weakly blocked RNA polymerase, while irradiation of 1 transformed the adducts into stronger blocks for RNA polymerase. The efficiency of irradiated 1 to form DNA interstrand cross-links was slightly greater than that of cisplatin in both 10 mM NaClO4 and 0.1 M NaCl. In contrast, the interstrand cross-linking efficiency of non-irradiated 1 in 10 mM NaClO4 was relatively low. An intermediate amount of cross-linking was observed when the sample of DNA already modified by non-irradiated 1 was irradiated. DNA unwinding measurements supported the conclusion that both mono- and bifunctional adducts with DNA can form. These results show that photoactivation of dinuclear Ru-II arene complexes can simultaneously produce a highly reactive ruthenium species that can bind to DNA and a fluorescent marker (the free arene). Importantly, the mechanism of photoreactivity is also independent of oxygen. These complexes, therefore, have the potential to combine both photoinduced cell death and fluorescence imaging of the location and efficiency of the photoactivation process.

ORCID iDs

Magennis, Steven W., Habtemariam, Abraha, Novakova, Olga, Henry, John B., Meier, Samuel, Parsons, Simon, Oswald, Iain D. H. ORCID logoORCID: https://orcid.org/0000-0003-4339-9392, Brabec, Viktor and Sadler, Peter J.;
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