Activity of immobilized papain dehydrated by n-propanol in low- water media

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Theppakorn, T. and Kanasawud, P. and Halling, P.J. (2004) Activity of immobilized papain dehydrated by n-propanol in low- water media. Biotechnology Letters, 26 (2). pp. 133-136. ISSN 0141-5492 (http://dx.doi.org/10.1023/B:BILE.0000012891.13904....)

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Abstract

A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degreesC. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h.

ORCID iDs

Theppakorn, T., Kanasawud, P. and Halling, P.J. ORCID logoORCID: https://orcid.org/0000-0001-5077-4088;
  • Item type:Article
    ID code:313
    Dates:
    Date
    Event
    January 2004
    Published
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user:Users 16 not found.
    Date deposited:10 Mar 2006
    Last modified:11 Nov 2024 08:26
    URI:https://strathprints.strath.ac.uk/id/eprint/313
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