Improving the penetration depth in multiphoton excitation laser scanning microscopy
McConnell, G. (2006) Improving the penetration depth in multiphoton excitation laser scanning microscopy. Journal of Biomedical Optics, 11 (5). ISSN 1083-3668 (http://dx.doi.org/10.1117/1.2360593)
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More than a threefold increase in multiphoton laser scanning microscopy depth penetration using a passive predispersion compensation system is reported. Using dispersion-controlled pulses to counteract the effects of positive group delay dispersion in the imaging platform, optical sectioning of fluorescent samples to depths in excess of 800 µm was observed, compared with only 240 µm using a noncompensated setup. Experimental results obtained from both the predispersion compensated and noncompensated systems are compared with theoretical values of pulse broadening in a laser scanning microscope. The observed improvement in depth profiling potentially widens the applications and user base of nonlinear microscopy techniques.
ORCID iDs
McConnell, G. ORCID: https://orcid.org/0000-0002-7213-0686;-
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Item type: Article ID code: 21245 Dates: DateEventSeptember 2006PublishedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Strathprints Administrator Date deposited: 16 Aug 2010 12:36 Last modified: 11 Nov 2024 09:30 URI: https://strathprints.strath.ac.uk/id/eprint/21245