Fluorescence nanotomography: a structural tool in biomedical sensing

Rolinski, O.J. and Birch, D.J.S. (2003) Fluorescence nanotomography: a structural tool in biomedical sensing. Proceedings of SPIE, 4876. pp. 836-847. ISSN 0277-786X (https://doi.org/10.1117/12.463935)

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Abstract

Fluorescence nanotomography (FN) is a newly developed method for determining molecular distributions on a nanometre scale in soft solids, biological macromolecules and medically important systems. FN uses fluorescence resonance energy transfer (FRET) for the recognition of the separations between molecules. By using a fluorescence lifetime measurement of sub-nanosecond time resolution, the spatial resolution of the resulting distribution function can be better than 1 �. In this paper the theoretical background of the method is outlined and the results of simulations on model molecular distributions presented. This is followed by demonstration of several applications of FN to real molecular systems, including bulk solutions of molecules of different sizes, complexes, porous polymers, phospholipids and sugar-protein competitive binding sensors glucose. The experimental requirements of FN as a structural tool for wide class of biomedical systems are discussed.

ORCID iDs

Rolinski, O.J. ORCID logoORCID: https://orcid.org/0000-0002-7838-779X and Birch, D.J.S. ORCID logoORCID: https://orcid.org/0000-0001-6400-1270;
  • Item type:Article
    ID code:18249
    Dates:
    Date
    Event
    27 August 2003
    Published
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Strathprints Administrator
    Date deposited:01 Apr 2010 19:24
    Last modified:11 Nov 2024 14:19
    URI:https://strathprints.strath.ac.uk/id/eprint/18249
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