Determination of acceptor distribution from fluorescence resonance energy transfer: theory and simulation

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Rolinski, O.J. and Birch, D.J.S. (2000) Determination of acceptor distribution from fluorescence resonance energy transfer: theory and simulation. Journal of Chemical Physics, 112 (20). pp. 8923-8933. ISSN 0021-9606 (http://dx.doi.org/10.1063/1.481506)

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Abstract

A new method for determining the donor-acceptor distribution function in fluorescence resonance energy transfer (FRET) systems is presented. The approach is based on time-resolved fluorescence experiments with nanosecond resolution. Potential applications of this method include: determination of the morphology of porous materials (e.g., polymers, sol-gels, resins, etc.), monitoring processes occurring on the nanometer scale including biomolecules labeled with the donor/acceptor species, and FRET sensors based on competitive binding. In this paper a theoretical derivation of the method is presented and the method is tested in a series of numerical simulations. The experimental conditions regarding this approach are discussed and its applicability to real measurement systems is demonstrated

ORCID iDs

Rolinski, O.J. ORCID logoORCID: https://orcid.org/0000-0002-7838-779X and Birch, D.J.S. ORCID logoORCID: https://orcid.org/0000-0001-6400-1270;
  • Item type:Article
    ID code:17495
    Dates:
    Date
    Event
    22 May 2000
    Published
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Strathprints Administrator
    Date deposited:05 May 2010 15:39
    Last modified:05 Dec 2024 04:38
    URI:https://strathprints.strath.ac.uk/id/eprint/17495
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