A method of determining donor-acceptor distribution functions in forster resonance energy transfer

Tools

Rolinski, O.J. and Birch, D.J.S. and McCartney, L.J. and Pickup, J.C. (2000) A method of determining donor-acceptor distribution functions in forster resonance energy transfer. Chemical Physics Letters, 324 (1-3). pp. 95-100. ISSN 0009-2614 (http://dx.doi.org/10.1016/S0009-2614(00)00577-7)

Full text not available in this repository.Request a copy

Abstract

We describe a method of determining the distribution function associated with acceptors partaking in Förster resonance energy transfer (FRET) from excited donors. Unlike previous approaches to this problem the method described makes no a priori assumption about the nature of the distribution. The use of an acceptor distribution function in analyte sensing is demonstrated for the first time. In the case of glucose sensing via FRET from Concanavalin A labelled with allophycocyanin to malachite green labelled dextran, the change in the acceptor distribution is shown to be a more sensitive sensing parameter than either the mean fluorescence lifetime or Förster γ value.

ORCID iDs

Rolinski, O.J. ORCID logoORCID: https://orcid.org/0000-0002-7838-779X, Birch, D.J.S. ORCID logoORCID: https://orcid.org/0000-0001-6400-1270, McCartney, L.J. and Pickup, J.C.;
  • Item type:Article
    ID code:17484
    Dates:
    Date
    Event
    30 June 2000
    Published
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Strathprints Administrator
    Date deposited:04 May 2010 15:42
    Last modified:11 Nov 2024 09:16
    URI:https://strathprints.strath.ac.uk/id/eprint/17484
CORE (COnnecting REpositories)