Attomole detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions

Wark, A.W. and Fang, S. and Lee, H.J. and Corn, R.M. (2006) Attomole detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions. Journal of American Chemical Society, 126. pp. 14044-14046. ISSN 0002-7863

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Abstract

Multiple microRNAs (miRNAs) are detected in a microarray format using a novel approach that combines a surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The surface reaction of poly(A) polymerase creates poly(A) tails on miRNAs hybridized onto locked nucleic acid (LNA) microarrays. DNA-modified nanoparticles are then adsorbed onto the poly(A) tails and detected with SPRI. This ultrasensitive nanoparticle-amplified SPRI methodology can be used for miRNA profiling at attomole levels.