Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa

Cornick, Jennifer and Musicha, Patrick and Peno, Chikondi and Seager, Ezgi and Tam, Pui Ying Iroh and Bilima, Sithembile and Bennett, Aisleen and Kennedy, Neil and Feasey, Nicholas and Heinz, Eva and Cain, Amy K. (2021) Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa. Microbial Genomics, 7 (11). 000703. ISSN 2057-5858 (https://doi.org/10.1099/mgen.0.000703)

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Abstract

A special-care neonatal unit from a large public hospital in Malawi was noted as having more frequent, difficult-to-treat infec-tions, and a suspected outbreak of multi-drug-resistant Klebsiella pneumoniae was investigated using genomic characterisa-tion. All K. pneumoniae bloodstream infections (BSIs) from patients in the neonatal ward (n=62), and a subset of K. pneumoniae BSI isolates (n=38) from other paediatric wards in the hospital, collected over a 4 year period were studied. After whole genome sequencing, the strain sequence types (STs), plasmid types, virulence and resistance genes were identified. One ST340 clone, part of clonal complex 258 (CC258) and an ST that drives hospital outbreaks worldwide, harbouring numerous resistance genes and plasmids, was implicated as the likely cause of the outbreak. This study contributes molecular information necessary for tracking and characterizing this important hospital pathogen in sub-Saharan Africa.

ORCID iDs

Cornick, Jennifer, Musicha, Patrick, Peno, Chikondi, Seager, Ezgi, Tam, Pui Ying Iroh, Bilima, Sithembile, Bennett, Aisleen, Kennedy, Neil, Feasey, Nicholas, Heinz, Eva ORCID logoORCID: https://orcid.org/0000-0003-4413-3756 and Cain, Amy K.;
  • Item type:Article
    ID code:90695
    Dates:
    Date
    Event
    18 November 2021
    Published
    5 October 2021
    Accepted
    Subjects:Science > Microbiology
    Department:UNSPECIFIED
    Depositing user: Pure Administrator
    Date deposited:24 Sep 2024 15:20
    Last modified:24 Sep 2024 15:20
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/90695
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