Pyocin S5 import into pseudomonas aeruginosa reveals a generic mode of bacteriocin transport

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Behrens, Hannah M. and Lowe, Edward D. and Gault, Joseph and Housden, Nicholas G. and Kaminska, Renata and Weber, T. Moritz and Thompson, Catriona M. A. and Mislin, Gaëtan L. A. and Schalk, Isabelle J. and Walker, Daniel and Robinson, Carol V. and Kleanthous, Colin (2020) Pyocin S5 import into pseudomonas aeruginosa reveals a generic mode of bacteriocin transport. mBio, 11 (2). pp. 1-16. ISSN 2150-7511 (https://doi.org/10.1128/mBio.03230-19)

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Abstract

Pyocin S5 (PyoS5) is a potent protein bacteriocin that eradicates the human pathogen Pseudomonas aeruginosa in animal infection models, but its import mechanism is poorly understood. Here, using crystallography, biophysical and biochemical analyses, and live-cell imaging, we define the entry process of PyoS5 and reveal links to the transport mechanisms of other bacteriocins. In addition to its C-terminal pore-forming domain, elongated PyoS5 comprises two novel tandemly repeated kinked 3-helix bundle domains that structure-based alignments identify as key import domains in other pyocins. The central domain binds the lipid-bound common polysaccharide antigen, allowing the pyocin to accumulate on the cell surface. The N-terminal domain binds the ferric pyochelin transporter FptA while its associated disordered region binds the inner membrane protein TonB1, which together drive import of the bacteriocin across the outer membrane. Finally, we identify the minimal requirements for sensitizing Escherichia coli toward PyoS5, as well as other pyocins, and suggest that a generic pathway likely underpins the import of all TonB-dependent bacteriocins across the outer membrane of Gram-negative bacteria.

ORCID iDs

Behrens, Hannah M., Lowe, Edward D., Gault, Joseph, Housden, Nicholas G., Kaminska, Renata, Weber, T. Moritz, Thompson, Catriona M. A., Mislin, Gaëtan L. A., Schalk, Isabelle J., Walker, Daniel ORCID logoORCID: https://orcid.org/0000-0002-4206-2942, Robinson, Carol V. and Kleanthous, Colin; Cossart, Pascale F.
  • Item type:Article
    ID code:86300
    Dates:
    Date
    Event
    28 April 2020
    Published
    28 January 2020
    Accepted
    11 December 2019
    Submitted
    Notes:© 2020 Behrens et al Behrens HM, Lowe ED, Gault J, Housden NG, Kaminska R, Weber TM, Thompson CMA, Mislin GLA, Schalk IJ, Walker D, Robinson CV, Kleanthous C. 2020. Pyocin S5 import into Pseudomonas aeruginosa reveals a generic mode of bacteriocin transport. mBio 11:e03230-19. https://doi.org/10.1128/mBio.03230-19
    Subjects:Science > Microbiology
    Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Depositing user: Pure Administrator
    Date deposited:27 Jul 2023 10:30
    Last modified:27 Nov 2024 01:23
    URI:https://strathprints.strath.ac.uk/id/eprint/86300
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