Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation
Muir, Rhona and Forbes, Shareen and Birch, David J S and Vyshemirsky, Vladislav and Rolinski, Olaf J (2023) Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation. Methods and Applications in Fluorescence, 11 (1). 015003. ISSN 2050-6120 (https://doi.org/10.1088/2050-6120/aca507)
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Abstract
We have studied the evolution of keratin intrinsic fluorescence as an indicator of its glycation. Steadystate and time-resolved fluorescence of free keratin and keratin-glucose samples were detected in PBS solutions in vitro. The changes in the fluorescence response demonstrate that the effect of glucose is manifest in the accelerated formation of fluorescent cross-links with an emission peak at 460 nm and formation of new cross-links with emission peaks at 525 nm and 575 nm. The fluorescence kinetics of these structures is studied and their potential application for the detection of long-term complications of diabetes discussed.
ORCID iDs
Muir, Rhona, Forbes, Shareen, Birch, David J S ORCID: https://orcid.org/0000-0001-6400-1270, Vyshemirsky, Vladislav and Rolinski, Olaf J ORCID: https://orcid.org/0000-0002-7838-779X;-
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Item type: Article ID code: 83343 Dates: DateEvent1 January 2023Published22 November 2022Published Online22 November 2022AcceptedSubjects: Science > Physics Department: Faculty of Science > Pure and Applied Chemistry
Technology and Innovation Centre > Bionanotechnology
Faculty of Science > PhysicsDepositing user: Pure Administrator Date deposited: 28 Nov 2022 10:51 Last modified: 04 Nov 2024 01:52 URI: https://strathprints.strath.ac.uk/id/eprint/83343