A smart self-balancing biosystem with reversible competitive adsorption of in-situ anion exchange resin for whole-cell catalysis preparation of lignocellulosic xylonic acid

Lv, Yang and Zhou, Shaonuo and Zhang, Xiaolei and Xu, Yong (2022) A smart self-balancing biosystem with reversible competitive adsorption of in-situ anion exchange resin for whole-cell catalysis preparation of lignocellulosic xylonic acid. Bioresource Technology, 363. 127998. ISSN 0960-8524 (https://doi.org/10.1016/j.biortech.2022.127998)

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Abstract

Xylonic acid (XA) bioproduction via whole-cell catalysis of Gluconobacter oxydans is a promising strategy for xylose bioconversion, which is hindered by inhibitor formation during lignocellulosic hydrolysates. Therefore, it is important to develop a catalytic system that can directly utilize hydrolysate and efficiently produce XA. Determination of the dynamic adsorption characteristics of 335 anion exchange resin resulted in a unique and interesting reversible competitive adsorption between acetic acid-like bioinhibitor, fermentable sugar and XA. Xylose in crude lignocellulosic hydrolysates was completely oxidized to 52.52 g/L XA in unprecedented self-balancing biological system through reversible competition. The obtained results showed that in-situ resin adsorption significantly affected the direct utilization of crude lignocellulosic hydrolysate for XA bioproduction (p ≤ 0.05). In addition, the resin adsorbed ca. 90 % of XA during bioconversion. The study achieved a multiple functions and integrated system, “detoxification, neutralization and product separation” for one-pot bioreaction of lignocellulosic hydrolysate.

ORCID iDs

Lv, Yang, Zhou, Shaonuo, Zhang, Xiaolei ORCID logoORCID: https://orcid.org/0000-0001-9415-3136 and Xu, Yong;