Engineered myoglobin as a catalyst for atom transfer radical cyclisation

Lubskyy, Andriy and Guo, Chao and Chadwick, Robert J. and Petri-Fink, Alke and Bruns, Nico and Pellizzoni, Michela M. (2022) Engineered myoglobin as a catalyst for atom transfer radical cyclisation. Chemical Communications, 58 (78). pp. 10989-10992. ISSN 1359-7345 (https://doi.org/10.1039/d2cc03227a)

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Abstract

Myoglobin was subjected to site-directed mutagenesis and transformed into a catalyst able to perform atom transfer radical cyclisation reactions, i.e. intramolecular atom transfer radical additions. Replacing the iron-coordinating histidine with serine, or introducing small changes inside or at the entrance of the active site, transformed the completely inactive wild-type myoglobin into an artificial metalloenzyme able to catalyse the 5-exo cyclisation of halogenated unsaturated compounds for the synthesis of γ-lactams. This new-to-nature activity was achieved not only with purified protein but also in crude cell lysate and in whole cells.

ORCID iDs

Lubskyy, Andriy, Guo, Chao, Chadwick, Robert J., Petri-Fink, Alke, Bruns, Nico ORCID logoORCID: https://orcid.org/0000-0001-6199-9995 and Pellizzoni, Michela M.;
  • Item type:Article
    ID code:82491
    Dates:
    Date
    Event
    31 August 2022
    Published
    31 August 2022
    Published Online
    30 August 2022
    Accepted
    Keywords:materials chemistry, metals and alloys, surfaces, coatings and films, general chemistry, ceramics and composites, electronic, optical and magnetic materials, catalysis, Chemistry, Chemical engineering, Materials Chemistry, Surfaces, Coatings and Films, Metals and Alloys, Ceramics and Composites, Chemistry(all), Catalysis, Electronic, Optical and Magnetic Materials
    Subjects:Science > Chemistry
    Technology > Chemical engineering
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:29 Sep 2022 14:43
    Last modified:05 Feb 2023 02:52
    URI:https://strathprints.strath.ac.uk/id/eprint/82491
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