Comparison of the antimicrobial efficacy and germicidal efficiency of 405-nm light for surface decontamination

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Sinclair, Lucy and MacGregor, Scott and Maclean, Michelle (2022) Comparison of the antimicrobial efficacy and germicidal efficiency of 405-nm light for surface decontamination. Access Microbiology, 4 (5). ISSN 2516-8290 (https://doi.org/10.1099/acmi.ac2021.po0226)

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Abstract

Background The persistence of infectious organisms on hospital surfaces presents a significant challenge to healthcare environments. Low irradiance visible 405-nm light has recently been developed as a method for environmental decontamination, with studies demonstrating successful reductions of environmental bacteria in wards and operating theatres. This study investigates the antimicrobial efficacy of 405-nm light for decontamination of surfaces, and how the dose-response kinetics are affected by use of differing light irradiances. Methods Surface-seeded Staphylococcus aureus and Pseudomonas aeruginosa (selected as model Gram-positive and Gram-negative species) were exposed to increasing doses of 405-nm light (≤ 90 Jcm-2) at three discrete irradiances (0.5, 5 and 50 mWcm-2). For both species, inactivation kinetics at each respective irradiance was established and susceptibility at equivalent light doses compared. Results Results demonstrate increased bacterial susceptibility to 405-nm light inactivation when exposed at lower irradiance treatments. For both species, 3 Jcm-2 was required when exposed using 0.5 mWcm-2 irradiance to achieve significant bacterial inactivation (P < 0.05; 26.7-73.7% reduction). When exposed at 5 mWcm-2, double the energy (6 Jcm-2) was required to achieve similar reductions. Exposure at the highest irradiance (50 mWcm-2) required 3-5 times greater dose (9-15 Jcm-2) to achieve similar reductions to the lowest irradiance tested (0.5 mWcm-2). Conclusion This study provides evidence of the enhanced germicidal efficiency of low irradiance 405-nm light, highlighting its efficacy for continuous environmental decontamination applications. Further investigation into the photo-chemical inactivation mechanisms will be crucial for its optimisation for a range of infection control applications.

ORCID iDs

Sinclair, Lucy ORCID logoORCID: https://orcid.org/0009-0007-8121-7795, MacGregor, Scott ORCID logoORCID: https://orcid.org/0000-0002-0808-585X and Maclean, Michelle ORCID logoORCID: https://orcid.org/0000-0001-5750-0397;
CORE (COnnecting REpositories)