Brain delivery of vasoactive intestinal peptide (VIP) following nasal administration to rats
Dufès, Christine and Olivier, Jean-Christophe and Gaillard, Frédéric and Gaillard, Afsaneh and Couet, William and Muller, Jean-Marc (2003) Brain delivery of vasoactive intestinal peptide (VIP) following nasal administration to rats. International Journal of Pharmaceutics, 255 (1-2). pp. 87-97. ISSN 1873-3476 (https://doi.org/10.1016/S0378-5173(03)00039-5)
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Abstract
The aim of this work was to study in rats the nasal route for the brain delivery of the vasoactive intestinal peptide (VIP) neuropeptide. After evaluating VIP stability in solutions obtained from nasal washes, the effect of formulation parameters (pH 4-9, 0-1% (w/v) lauroylcarnitine (LC), hypo- or isoosmolality) on the brain uptake of intranasally administered VIP (10(-8)M)/125I-VIP (300,000 cpm/ml) was studied, using an in situ perfusion technique. Brain radioactivity distribution was assessed by quantitative autoradiographic analysis. Results were compared to intravenously administered VIP. With a hypotonic formulation at pH 4 containing 0.1% LC and 1% bovine serum albumin, VIP stability was satisfactory and loss by adsorption was minimal. Using this formulation, around 0.11% of initial radioactivity was found in the brain after 30 min perfusion and was located in the olfactory bulbs, the midbrain and the cerebellum. HPLC analysis of brain and blood extracts demonstrated the presence of intact VIP in brain and its complete degradation in the blood compartment. By intravenous administration, no intact VIP was found either in brain or in blood. In conclusion, intact VIP could be delivered successfully to the brain using the intranasal route for administration.
ORCID iDs
Dufès, Christine ORCID: https://orcid.org/0000-0002-7963-6364, Olivier, Jean-Christophe, Gaillard, Frédéric, Gaillard, Afsaneh, Couet, William and Muller, Jean-Marc;-
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Item type: Article ID code: 7828 Dates: DateEvent2003PublishedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Strathprints Administrator Date deposited: 03 Apr 2009 13:05 Last modified: 23 Nov 2024 01:02 URI: https://strathprints.strath.ac.uk/id/eprint/7828