Adaptation to endophytic lifestyle through genome reduction by Kitasatospora sp. SUK42
Zin, Noraziah M. and Ismail, Aisha and Mark, David R. and Westrop, Gareth and Schniete, Jana K. and Herron, Paul (2021) Adaptation to endophytic lifestyle through genome reduction by Kitasatospora sp. SUK42. Frontiers in Bioengineering and Biotechnology, 9. 740722. ISSN 2296-4185 (https://doi.org/10.3389/fbioe.2021.740722)
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Abstract
Endophytic actinobacteria offer great potential as a source of novel bioactive compounds. In order to investigate the potential for the production of secondary metabolites by endophytes, we recovered a filamentous microorgansism from the tree Antidesma neurocarpum Miq. After phenotypic analysis and whole genome sequencing we demonstrated that this organism, SUK42 was a member of the actinobacterial genus Kitasatospora. This strain has a small genome in comparison with other type strains of this genus and has lost metabolic pathways associated with Stress Response, Nitrogen Metabolism and Secondary Metabolism. Despite this SUK42 can grow well in a laboratory environment and encodes a core genome that is consistent with other members of the genus. Finally, in contrast to other members of Kitasatospora, SUK42 encodes saccharide secondary metabolite biosynthetic gene clusters, one of which with similarity to the acarviostatin cluster, the product of which displays α-amylase inhibitory activity. As extracts of the host plant demonstrate this inhibitory activity, it suggests that the potential medicinal properties of A. neurocarpum Miq might be provided by the endophytic partner and illustrate the potential for exploitation of endophytes for clinical or industrial uses.
ORCID iDs
Zin, Noraziah M., Ismail, Aisha, Mark, David R., Westrop, Gareth ORCID: https://orcid.org/0000-0002-8011-6219, Schniete, Jana K. ORCID: https://orcid.org/0000-0003-4274-5839 and Herron, Paul ORCID: https://orcid.org/0000-0003-3431-1803;-
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Item type: Article ID code: 78239 Dates: DateEvent12 October 2021Published12 October 2021Published Online20 September 2021Accepted13 July 2021SubmittedSubjects: Science > Microbiology Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 21 Oct 2021 10:45 Last modified: 11 Nov 2024 13:09 URI: https://strathprints.strath.ac.uk/id/eprint/78239