Rational design of adjuvants for subunit vaccines : the format of cationic adjuvants affects the induction of antigen-specific antibody responses
Anderluzzi, Giulia and Schmidt, Signe Tandrup and Cunliffe, Robert and Woods, Stuart and Roberts, Craig W. and Veggi, Daniele and Ferlenghi, Ilaria and O'Hagan, Derek T. and Baudner, Barbara C. and Perrie, Yvonne (2021) Rational design of adjuvants for subunit vaccines : the format of cationic adjuvants affects the induction of antigen-specific antibody responses. Journal of Controlled Release, 330. pp. 933-944. ISSN 0168-3659 (https://doi.org/10.1016/j.jconrel.2020.10.066)
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Abstract
A range of cationic delivery systems have been investigated as vaccine adjuvants, though few direct comparisons exist. To investigate the impact of the delivery platform, we prepared four cationic systems (emulsions, liposomes, polymeric nanoparticles and solid lipid nanoparticles) all containing equal concentrations of the cationic lipid dimethyldioctadecylammonium bromide in combination with the Neisseria adhesin A variant 3 subunit antigen. The formulations were physicochemically characterized and their ability to associate with cells and promote antigen processing (based on degradation of DQ-OVA, a substrate for proteases which upon hydrolysis is fluorescent) was compared in vitro and their vaccine efficacy (antigen-specific antibody responses and IFN-γ production) and biodistribution (antigen and adjuvant) were evaluated in vivo. Due to their cationic nature, all delivery systems gave high antigen loading (> 85%) with liposomes, lipid nanoparticles and emulsions being <200 nm, whilst polymeric nanoparticles were larger (~350 nm). In vitro, the particulate systems tended to promote cell uptake and antigen processing, whilst emulsions were less effective. Similarly, whilst the particulate delivery systems induced a depot (of both delivery system and antigen) at the injection site, the cationic emulsions did not. However, out of the systems tested the cationic emulsions induced the highest antibody responses. These results demonstrate that while cationic lipids can have strong adjuvant activity, their formulation platform influences their immunogenicity.
ORCID iDs
Anderluzzi, Giulia, Schmidt, Signe Tandrup, Cunliffe, Robert, Woods, Stuart ORCID: https://orcid.org/0000-0002-3798-2074, Roberts, Craig W. ORCID: https://orcid.org/0000-0002-0653-835X, Veggi, Daniele, Ferlenghi, Ilaria, O'Hagan, Derek T., Baudner, Barbara C. and Perrie, Yvonne;-
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Item type: Article ID code: 76003 Dates: DateEvent10 February 2021Published2 November 2020Published Online30 October 2020AcceptedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 01 Apr 2021 14:20 Last modified: 13 Sep 2024 00:37 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/76003