Theoretical investigations of a modified compressed ultrafast photography method suitable for single-shot fluorescence lifetime imaging

Li, Yahui and Tian, Jinshou and Li, David Day-Uei (2021) Theoretical investigations of a modified compressed ultrafast photography method suitable for single-shot fluorescence lifetime imaging. Applied Optics, 60 (5). 1476-1483. ISSN 1559-128X (https://doi.org/10.1364/AO.415594)

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Abstract

A single-shot fluorescence lifetime imaging (FLIM) method based on the compressed ultrafast photog- raphy (CUP) is proposed, named space-restricted CUP (srCUP). srCUP is suitable for imaging objects moving slowly (< ∼ 150/M mm/s, M is the magnification of the objective lens) in the field of view with the intensity changing within nanoseconds in a measurement window around 10 ns. We used synthetic datasets to explore the performances of srCUP compared with CUP and TCUP (a variant of CUP). srCUP not only provides superior reconstruction performances, but its reconstruction time is also two- and three- fold faster than CUP and TCUP, respectively. The lifetime determination performances were assessed by estimating lifetime components, amplitude- and intensity-weighted average lifetimes (τA and τI) with the reconstructed scenes using the least square method based on a bi-exponential model. srCUP has the best accuracy and precision for lifetime determinations with a relative bias less than 7% and a coefficient of variation less than 7% for τA and a relative bias less than 10% and a coefficient of variation less than 11% for τI.

ORCID iDs

Li, Yahui, Tian, Jinshou and Li, David Day-Uei ORCID logoORCID: https://orcid.org/0000-0002-6401-4263;
CORE (COnnecting REpositories)