A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

Rodríguez, Jéssica and Mosquera, Jesús and García-Fandiño, Rebeca and Eugenio Vázquez, M. and Mascareñas, José L. (2016) A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves. Chemical Science, 7 (5). pp. 3298-3303. ISSN 2041-6520

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    Abstract

    We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).