Tracking insulin glycation in real time by time-resolved emission spectroscopy
Chung, Li Hung C. and Birch, David J. S. and Vyshemirsky, Vladislav and Ryadnov, Maxim G. and Rolinski, Olaf J. (2019) Tracking insulin glycation in real time by time-resolved emission spectroscopy. Journal of Physical Chemistry B, 123 (37). pp. 7812-7817. ISSN 1520-6106 (https://doi.org/10.1021/acs.jpcb.9b06363)
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Abstract
The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.
ORCID iDs
Chung, Li Hung C. ORCID: https://orcid.org/0000-0002-1043-3227, Birch, David J. S. ORCID: https://orcid.org/0000-0001-6400-1270, Vyshemirsky, Vladislav, Ryadnov, Maxim G. and Rolinski, Olaf J. ORCID: https://orcid.org/0000-0002-7838-779X;-
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Item type: Article ID code: 72085 Dates: DateEvent19 September 2019Published23 August 2019Published Online14 August 2019AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Physics
Technology and Innovation Centre > BionanotechnologyDepositing user: Pure Administrator Date deposited: 17 Apr 2020 12:26 Last modified: 11 Nov 2024 12:39 URI: https://strathprints.strath.ac.uk/id/eprint/72085