Biocatalytic ATRP in solution and on surfaces

Rodriguez, Kyle J. and Pellizzoni, Michela M. and Divandari, Mohammad and Benetti, Edmondo M. and Bruns, Nico; Bruns, Nico and Loos, Katja, eds. (2019) Biocatalytic ATRP in solution and on surfaces. In: Methods in Enzymology. Methods in Enzymology, 627 . Academic Press Inc., Cambridge, MA, pp. 263-290. ISBN 9780128170953 (

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The promiscuity of enzymes allows for their implementation as catalysts for non-native chemical transformations. Utilizing the redox activity of metalloenzymes under activator regenerated by electron transfer (ARGET) ATRP conditions, well-controlled and defined polymers can be generated. In this chapter, we review bioATRP in solution and on surfaces and provide experimental protocols for hemoglobin-catalyzed ATRP and for surface-initiated biocatalytic ATRP. This chapter highlights the polymerization of acrylate and acrylamide monomers and provides detailed experimental protocols for the characterization of the polymers and of the polymer brushes.