Superior biological performance and osteoinductive activity of Si-containing bioactive bone regeneration particles for alveolar bone reconstruction

Mao, Zhe and Gu, Yifan and Zhang, Jing and Shu, Will Wenmiao and Cui, Yingqiu and Xu, Tao (2020) Superior biological performance and osteoinductive activity of Si-containing bioactive bone regeneration particles for alveolar bone reconstruction. Ceramics International, 46 (1). pp. 353-364. ISSN 0272-8842 (https://doi.org/10.1016/j.ceramint.2019.08.269)

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Abstract

Bone grafting materials for repair of alveolar bone deficits have improved markedly in recent years, increasing the applicability and success of oral implantology. The long-term success rate of dental implant surgery is strongly dependent on the quality and stability of residual bone tissue. Therefore, reconstruction of resorbed alveolar bone is a challenge for clinicians. In the present study, we have developed bioactive bone regeneration particles (BRPs) using amorphous calcium phosphate and 58S bioglass as raw materials. The structural characteristics, biocompatibility, and osteoinductivity of these BRPs were compared to commercially available bovine spongy bone (BSB) without organic components. X-ray diffractometry (XRD) and scanning electron microscopy (SEM) showed that BRPs were composed of β-tricalcium phosphate (β-TCP) and calcium silicate in the form of hexagonal crystals, while BSB was mainly hydroxyapatite (HA) arranged in orderly nano-sized crystals. The viability of human bone marrow mesenchymal stem cells (hBMSCs) cultured in BRP-containing medium was roughly equal to that of hBMSCs in control medium. Moreover, hBMSCs in BRP medium exhibited greater proliferation rates, substrate attachment, alkaline phosphatase (ALP) activity, alizarin red staining intensity, and expression levels of osteogenic-related genes (COL-I, OCN, Runx-2, ALP, BSP) than hBMSCs in BSB medium, indicating the superior osteoinductivity of BRPs. Silicon ions released from BRPs during cell culture were crucial for these enhanced biological properties. BRPs also demonstrated superior osteoconduction and osteoinduction properties for bone defect repair, suggesting promise for alveolar bone repair surgery.

  • Item type:Article
    ID code:70436
    Dates:
    Date
    Event
    1 January 2020
    Published
    29 August 2019
    Published Online
    27 August 2019
    Accepted
    Keywords:alveolar bone reconstruction, bioactive bone graft, osteoconduction, osteoinduction, Bioengineering, Electronic, Optical and Magnetic Materials, Ceramics and Composites, Process Chemistry and Technology, Surfaces, Coatings and Films, Materials Chemistry
    Subjects:Technology > Engineering (General). Civil engineering (General) > Bioengineering
    Department:Faculty of Engineering > Biomedical Engineering
    Depositing user: Pure Administrator
    Date deposited:06 Nov 2019 12:12
    Last modified:05 Dec 2022 04:13
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/70436
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