Microfluidic-assisted silk nanoparticle tuning
Wongpinyochit, Thidarat and Totten, John D. and Johnston, Blair F. and Seib, F. Philipp (2019) Microfluidic-assisted silk nanoparticle tuning. Nanoscale Advances, 1 (2). pp. 873-883.
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Abstract
Silk is now making inroads into advanced pharmaceutical and biomedical applications. Both bottom-up and top-down approaches can be applied to silk and the resulting aqueous silk solution can be processed into a range of material formats, including nanoparticles. Here, we demonstrate the potential of microfluidics for the continuous production of silk nanoparticles with tuned particle characteristics. Our microfluidic-based design ensured efficient mixing of different solvent phases at the nanoliter scale, in addition to controlling the solvent ratio and flow rates. The total flow rate and aqueous : solvent ratios were important parameters affecting yield (1 mL min−1 > 12 mL min−1). The ratios also affected size and stability; a solvent : aqueous total flow ratio of 5 : 1 efficiently generated spherical nanoparticles 110 and 215 nm in size that were stable in water and had a high beta-sheet content. These 110 and 215 nm silk nanoparticles were not cytotoxic (IC50 > 100 μg mL−1) but showed size-dependent cellular trafficking. Overall, microfluidic-assisted silk nanoparticle manufacture is a promising platform that allows control of the silk nanoparticle properties by manipulation of the processing variables.
Creators(s): |
Wongpinyochit, Thidarat ![]() ![]() ![]() ![]() | Item type: | Article |
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ID code: | 66520 |
Keywords: | silk, nanoparticle tuning, pharmaceutical applications of silk, Pharmacy and materia medica, Pharmacology, Toxicology and Pharmaceutics(all) |
Subjects: | Medicine > Pharmacy and materia medica |
Department: | Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Technology and Innovation Centre > Bionanotechnology |
Depositing user: | Pure Administrator |
Date deposited: | 10 Jan 2019 12:24 |
Last modified: | 21 Jan 2021 10:39 |
URI: | https://strathprints.strath.ac.uk/id/eprint/66520 |
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