Picture of boy being examining by doctor at a tuberculosis sanatorium

Understanding our future through Open Access research about our past...

Strathprints makes available scholarly Open Access content by researchers in the Centre for the Social History of Health & Healthcare (CSHHH), based within the School of Humanities, and considered Scotland's leading centre for the history of health and medicine.

Research at CSHHH explores the modern world since 1800 in locations as diverse as the UK, Asia, Africa, North America, and Europe. Areas of specialism include contraception and sexuality; family health and medical services; occupational health and medicine; disability; the history of psychiatry; conflict and warfare; and, drugs, pharmaceuticals and intoxicants.

Explore the Open Access research of the Centre for the Social History of Health and Healthcare. Or explore all of Strathclyde's Open Access research...

Image: Heart of England NHS Foundation Trust. Wellcome Collection - CC-BY.

An organic semiconductor laser platform for the detection of DNA by AgNP plasmonic enhancement

McConnell, G. and Mabbott, S. and Kanibolotskyy, A. L. and Skabara, P. J. and Graham, D. and Burley, G. A. and Laurand, N. (2018) An organic semiconductor laser platform for the detection of DNA by AgNP plasmonic enhancement. Langmuir. ISSN 0743-7463

[img]
Preview
Text (McConnell-etal-Langmuir-2018-An-organic-semiconductor-laser-platform-for-the-detection-of-DNA)
McConnell_etal_Langmuir_2018_An_organic_semiconductor_laser_platform_for_the_detection_of_DNA.pdf
Final Published Version
License: Creative Commons Attribution 4.0 logo

Download (2MB) | Preview

Abstract

Organic semiconductor lasers are a sensitive biosensing platform that responds to specific biomolecule binding event. So far, such biosensors have utilized protein-based interactions for surface functionalization but a nucleic acid-based strategy would considerably widen their utility as a general biodiagnostic platform. This manuscript reports two important advances for DNA-based sensing using an organic semiconductor (OS) distributed feedback (DFB) laser. Firstly, the immobilization of alkyne-tagged 12/18-mer oligodeoxyribonucleotide (ODN) probes by Cu-catalyzed azide alkyne cycloaddition (CuAAC) or ‘click chemistry’ onto an 80-nm thick OS laser film modified with an azide-presenting polyelectrolyte monolayer is presented. Secondly, sequence-selective binding to these immobilized probes with complementary ODN functionalized silver nanoparticles, is detected. As binding occurs, the nanoparticles increase the optical losses of the laser mode through plasmonic scattering and absorption, and this causes a rise in the threshold pump energy required for laser action that is proportional to the analyte concentration. By monitoring this threshold, detection of the complementary ODN target down to 11.5 pM is achieved. This complementary binding on the laser surface is independently confirmed through surface-enhanced Raman spectroscopy (SERS).