Light-sheet microscopy beam path miniaturisation using optical MEMS

Bauer, R. and Bakas, S. and Uttamchandani, D. (2018) Light-sheet microscopy beam path miniaturisation using optical MEMS. In: Photon 18, 2018-09-03 - 2018-09-06, Ashton University.

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Abstract

Light-sheet microscopy (LSM) as 3D fluorescence imaging tool has shown impressive growth over the last decade, specifically with open-source and application tailored systems in mind. By structuring the fluorescence excitation light to only illuminate a thin sheet of the sample in the active focal plane of an orthogonal imaging path, low light doses and fast 3D imaging can be achieved. In this work, we present digital control of the light-sheet creation, positioning and imaging using Microelectromechanical Systems (MEMS) micromirrors and the use of their inherent miniaturisation potential.

ORCID iDs

Bauer, R. ORCID logoORCID: https://orcid.org/0000-0001-7927-9435, Bakas, S. and Uttamchandani, D. ORCID logoORCID: https://orcid.org/0000-0002-2362-4874;
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