Picture of DNA strand

Pioneering chemical biology & medicinal chemistry through Open Access research...

Strathprints makes available scholarly Open Access content by researchers in the Department of Pure & Applied Chemistry, based within the Faculty of Science.

Research here spans a wide range of topics from analytical chemistry to materials science, and from biological chemistry to theoretical chemistry. The specific work in chemical biology and medicinal chemistry, as an example, encompasses pioneering techniques in synthesis, bioinformatics, nucleic acid chemistry, amino acid chemistry, heterocyclic chemistry, biophysical chemistry and NMR spectroscopy.

Explore the Open Access research of the Department of Pure & Applied Chemistry. Or explore all of Strathclyde's Open Access research...

Resonance Raman detection of antioxidants using an iron oxide nanoparticle catalysed decolourisation assay

Sloan-Dennison, Sian and Shand, Neil C. and Graham, Duncan and Faulds, Karen (2017) Resonance Raman detection of antioxidants using an iron oxide nanoparticle catalysed decolourisation assay. Analyst, 142 (24). pp. 4715-4720. ISSN 0003-2654

[img]
Preview
Text (Sloan-Dennison-etal-Analyst-2017-Resonance-Raman-detection-of-antioxidants-using-an-iron-oxide-nanoparticle)
Sloan_Dennison_etal_Analyst_2017_Resonance_Raman_detection_of_antioxidants_using_an_iron_oxide_nanoparticle.pdf
Accepted Author Manuscript

Download (825kB) | Preview

Abstract

Nanozymes are metal nanoparticles with catalytic properties that can be used to oxidise peroxidase substrates giving a colorimetric response which can be detected using UV-vis, and recently, Raman spectroscopy. Due to their ease of synthesis and increased stability, nanozymes are being increasing investigated to replace conventional enzymes for the detection of biomolecules. Here we exploit the catalytic activity of iron oxide (Fe2O3) nanoparticles combined with the substrate 2,2-Azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) in a decolourisation assay for the detection of antioxidants. Fe2O3 nanoparticles were used to catalyse the oxidation of ABTS to its green radical cation which, upon the addition of an antioxidant, resulted in a decolourisation due to the reduction of the radical cation caused by the hydrogen donating antioxidant. The assay was applied for the detection of multiple antioxidants (glutathione, chlorogenic acid and ascorbic acid), and was followed by monitoring the resonance Raman scattering from the ABTS solution using a portable Raman system with 785 nm laser excitation. This novel assay has the potential to be optimised to detect antioxidant activity in body fluid with low limits of detection with point of use monitoring.