Synthetic biological approaches for RNA labelling and imaging : design principles and future opportunities

Pauff, Steven and Withers, Jamie M and McKean, Iain JW and Mackay, Simon P and Burley, Glenn A (2017) Synthetic biological approaches for RNA labelling and imaging : design principles and future opportunities. Current Opinion in Biotechnology, 48. pp. 153-158. ISSN 0958-1669

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    Abstract

    RNA is the most mercurial of all biomacromolecules. In contrast to DNA, where the predominant role is the storage of genetic information, the biological role of RNA varies; ranging from a template-based intermediary in gene expression to playing a direct role in catalysis. Their high turnover and metabolic lability makes the detection of specific sequences particularly challenging. This review describes the latest synthetic biological developments that enable the direct imaging of RNA both in vitro and in their native cellular environment.

    Author(s):Pauff, Steven ORCID logoORCID: https://orcid.org/0000-0002-7499-3207, Withers, Jamie M ORCID logoORCID: https://orcid.org/0000-0002-9746-9467, McKean, Iain JW ORCID logoORCID: https://orcid.org/0000-0002-4970-1508, Mackay, Simon P ORCID logoORCID: https://orcid.org/0000-0001-8000-6557 and Burley, Glenn A
    Item type:Article
    ID code:61978
    Notes:Published under Crown Copyright (Open Government Licence 3.0).
    Keywords:RNA, biomacromolecules, RNA imaging, Chemistry, Microbiology, Biotechnology, Bioengineering, Biomedical Engineering
    Subjects:Science > Chemistry
    Science > Microbiology
    Department:Faculty of Science > Pure and Applied Chemistry
    Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:09 Oct 2017 14:54
    Last modified:04 Aug 2019 00:44
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/61978
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