From single-molecule spectroscopy to super-resolution imaging of the neuron : a review

Laine, Romain F and Schierle, Gabriele S Kaminski and van de Linde, Sebastian and Kaminski, Clemens F (2016) From single-molecule spectroscopy to super-resolution imaging of the neuron : a review. Methods and Applications in Fluorescence, 4 (2). 022004. ISSN 2050-6120 (https://doi.org/10.1088/2050-6120/4/2/022004)

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Abstract

For more than 20 years, single-molecule spectroscopy has been providing invaluable insights into nature at the molecular level. The field has received a powerful boost with the development of the technique into super-resolution imaging methods, ca. 10 years ago, which overcome the limitations imposed by optical diffraction. Today, single molecule super-resolution imaging is routinely used in the study of macromolecular function and structure in the cell. Concomitantly, computational methods have been developed that provide information on numbers and positions of molecules at the nanometer-scale. In this overview, we outline the technical developments that have led to the emergence of localization microscopy techniques from single-molecule spectroscopy. We then provide a comprehensive review on the application of the technique in the field of neuroscience research.

  • Item type:Article
    ID code:61351
    Dates:
    Date
    Event
    24 June 2016
    Published
    19 May 2016
    Accepted
    Subjects:Science
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:25 Jul 2017 11:36
    Last modified:13 Apr 2024 00:23
    URI:https://strathprints.strath.ac.uk/id/eprint/61351
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