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Literary linguistics: Open Access research in English language

Strathprints makes available Open Access scholarly outputs by English Studies at Strathclyde. Particular research specialisms include literary linguistics, the study of literary texts using techniques drawn from linguistics and cognitive science.

The team also demonstrates research expertise in Renaissance studies, researching Renaissance literature, the history of ideas and language and cultural history. English hosts the Centre for Literature, Culture & Place which explores literature and its relationships with geography, space, landscape, travel, architecture, and the environment.

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Acanthamoeba activates macrophages predominantly through toll-like receptor 4 and MyD88-dependent mechanisms to induce Interleukin IL-12 and IL-6

Cano, Antonella and Mattana, Antonella and Woods, Stuart and Henriquez, Fiona L. and Alexander, James and Roberts, Craig W. (2017) Acanthamoeba activates macrophages predominantly through toll-like receptor 4 and MyD88-dependent mechanisms to induce Interleukin IL-12 and IL-6. Infection and Immunity, 85 (6). ISSN 0019-9567

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Abstract

Acanthamoeba castellanii is a free-living ubiquitous amoeba, with a worldwide distribution, that can occasionally infect humans, causing particularly severe infections in immune compromised individuals. Dissecting the immunology of Acanthamoeba infections has been considered problematic due to the very low incidence of disease despite the high exposure rates. Whilst macrophages are acknowledged as playing a significant role in Acanthamoeba infections little is known about how this facultative parasite influences macrophage activity. Therefore, in this study we investigate the effects of Acanthamoeba on the activation of resting macrophages. Consequently, murine bone marrow derived macrophages were co-cultured with trophozoites of either the laboratory Neff strain, or a clinical isolate of A. castellanii. In vitro real-time imaging demonstrated that trophozoites of both strains often established evanescent contact with macrophages. Both Acanthamoeba strains induced a pro-inflammatory macrophage phenotype characterized by significant production of IL-12 and IL-6. However, macrophages co-cultured with the clinical isolate of Acanthamoeba produced significantly less IL-12 and IL-6 in comparison to the Neff strain. The utilization of macrophages derived from MyD88, TRIF, TLR2, TLR4, TLR2/4 deficient mice indicated that Acanthamoeba-induced pro-inflammatory cytokine production was through MyD88-dependent, TRIF independent, TLR4-induced events. This study shows for the first time the involvement of TLRs, expressed on macrophages in the recognition and response to Acanthamoeba trophozoites.