Light-triggered inactivation of enzymes with photothermal nanoheaters

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Thompson, Sebastian A. and Paterson, Sureyya and Azab, Marwa M. M. and Wark, Alastair W. and de la Rica, Roberto (2017) Light-triggered inactivation of enzymes with photothermal nanoheaters. Small. 1603195. ISSN 1613-6810 (https://doi.org/10.1002/smll.201603195)

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Abstract

Enzymes are crucial for the cell metabolism and in industrial applications such as the manufacture of biosensors and medicines. A method for inactivating enzymes non-invasively and on demand could reveal the role of a biocatalyst in a particular cell function and enable fine-tuning the therapeutic dose of enzyme-based drugs. In this manuscript we introduce a universal method for inactivating enzymes with light. It consists of attaching the enzymes to photothermal nanoheaters (gold nanorods). Upon irradiation with light resonant with their LSPR the plasmonic nanoparticles generate heat, which denatures the enzymes. The resulting increase in temperature is highest in the vicinity of the nanoheaters, which allows the localized inactivation of nanorod-bound enzymes without damaging other proteins or cell membranes present in the same solution. Here we demonstrate this concept by selectively turning off an enzyme cascade via the selective inactivation of one of its components. We also prove that the target enzyme can be inactivated without unduly damaging cells under controlled irradiation conditions. The results shown here pave the way for using the proposed methodology to discern the role of target enzymes in intracellular signaling pathways.

ORCID iDs

Thompson, Sebastian A., Paterson, Sureyya ORCID logoORCID: https://orcid.org/0000-0001-5433-7811, Azab, Marwa M. M., Wark, Alastair W. ORCID logoORCID: https://orcid.org/0000-0001-8736-7566 and de la Rica, Roberto;
  • Item type:Article
    ID code:59696
    Dates:
    Date
    Event
    2 February 2017
    Published
    2 February 2017
    Accepted
    Notes:This is the peer reviewed version of the following article: Thompson, S. A., Paterson, S., Azab, M. M. M., Wark, A. W., & de la Rica, R. (2017). Light-triggered inactivation of enzymes with photothermal nanoheaters. Small, 1-23., which has been published in final form at 10.1002/smll.201603195. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:03 Feb 2017 12:29
    Last modified:11 Nov 2024 11:37
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/59696
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