Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA

Weldon, Carika and Behm-Ansmant, Isabelle and Hurley, Laurence H and Burley, Glenn A and Branlant, Christiane and Eperon, Ian C and Dominguez, Cyril (2016) Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA. Nature Chemical Biology, 13. pp. 18-20. ISSN 1552-4450

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    Abstract

    RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.